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These lines are the result of an extensive pedigree selection methodology including thousands of lines and over four years of nursery line screening as well as early and late generation hybrid testing across locations, years, and industry testers. Two of the new inbred lines seem promising for use as parents for 70 to 85RM hybrids (Set 1) while four new inbred lines seem promising for use as parents for 85 to 95RM hybrids (Set 2). Set 1 Experimental inbred ND00-24 is proposed as ND2006. The average performance across locations of single crosses that included ND00-24 as one parent was 13.24% for protein grain content, 56.92 lb/Bu test weight, and 17.46% grain moisture at harvest compared with the average 11.60% for protein grain content, 56.40 lb/Bu test weight, and 18.33% grain moisture at harvest for the commercial check hybrids. It combines best with Iodent and LH82-derived testers. It has shown good combining ability with B14 under drought stress in western ND. Experimental inbred ND99-8 is proposed as ND2005. The average performance across locations of single crosses that included ND99-8 as one parent was 57.23 lb/Bu test weight, and 17.75% grain moisture at harvest compared with the average, 56.40 lb/Bu for test weight and 18.33% grain moisture at harvest for the commercial check hybrids. Average performance of ND99-8 for other agronomic traits was similar; except ND99-8 crosses had 2.89% less stalk lodging and 9.27% more root lodging. It combines best with Iodent and LH82-derived testers. Experimental inbred ND01-27 is proposed as ND2004. Average yield across locations of single crosses that included ND01-27 as one parent was 74.1 q ha^-1 (117.85 Bu/A) compared with the average yield of 72.0 q ha^-1 (114.51 Bu/A) for the commercial check hybrids. Average performance of ND01-27 for other agronomic traits was similar, except ND01-27 crosses had 6.2% less root lodging and 3.3% less stalk lodging. It combines well across testers of several heterotic groups. Experimental inbred ND00-50 is proposed as ND2003. This line had the best specific combining ability for grain yield across locations (80.8 q ha^-1 or 128.43 Bu/A) in combination with commercial tester TR3621Bt especially under irrigated conditions. It combines well across testers of several heterotic groups. Experimental inbred ND01-32 is proposed as ND2002. Average yield across locations of single crosses that included ND01-27 as one parent was 76.4 q ha^-1 (121.50 Bu/A) compared with the average yield of 72.0 q ha^-1 (114.51 Bu/A) for the commercial check hybrids. Average performance of ND01-32 for other agronomic traits was similar, except ND01-32 crosses had 5.3% greater root lodging and 2.5 lb/Bu less test weight. It combines best with Iodent and LH82-derived testers especially under irrigation. Experimental inbred ND01-4 is proposed as ND2001. Average yield across locations of single crosses that included ND01-4 as one parent was 74.6 q ha^-1 (118.60 Bu/A) compared with the average yield of 72.0 q ha^-1 (114.51 Bu/A) for the commercial check hybrids. Average performance of ND01-4 for other agronomic traits was similar, except ND01-4 crosses had 2.7 lb/Bu less test weight. It combines best with LH82-derived testers especially in western ND. Registration of ND291 Inbred Line of Maize Inbred ND291 (Reg. No. PL-305, PI 631393) is yellow-dent maize (Zea mays L.) inbred line developed at the North Dakota Agricultural Experiment Station, North Dakota State University (NDSU), Fargo, ND. ND291 was released 15 February 2002 because of its potential value as germplasm source in pedigree-selection breeding programs for the northern Corn Belt. ND291 was selected from NDSM(M)C1, a strain of NDSM that has undergone mass selection for increased grain yield and reduced stalk and root lodging. Thirteen inbred lines of approximately AES100 to AES300 maturity were intercrossed to develop NDSM (Cross and Wanner, 1991). These inbred lines were chosen for good general combining ability for grain yield and stalk lodging resistance. The original S₀ plant was selected from an isolated planting of NDSM(M)C1 and advanced ear-to-row by five generations of self-pollination. ND291 [NDSM(M)C1-1-1-1-1-1-2] was identified in a testcross with an early B14 tester (CM105 x A665) at the S₆ generation. Late-generation testing of ND291 was performed in 1996 (three locations), 1998 (five locations), 1999 (one location), 2000 (six locations), and 2001 (six locations) with commercial check hybrids (2 to 5) included in each trial. The average grain yield of two and three-way hybrid combinations with ND291 (7.45 t ha^-1) across environments were statistically (P< 0.05) similar to commercial check hybrids (7.36 t ha^-1). Crosses that included ND291 had also statistically similar grain moisture at harvest (181.0 g kg^-1 vs. 171.0 g kg^-1), similar root lodging (3.7% vs. 3.2%), and similar stalk lodging (4.0% vs. 3.0%). ND291 hybrids had 10 g kg^-1 greater protein concentration and equal starch and oil concentration than commercial checks. ND291 has shown good combining ability with early Iowa Stiff Stalk Synthetic (BSSS) and unrelated testers. Simple sequence repeats (SSRs) have suggested that ND291 could be assigned to the BSSS heterotic group. Grain yield data, however, do not support molecular data. ND291 is a vigorous line with above average seedling emergence percentage in cold soils. It has average resistance to early infection by eyespot (caused by Kabatiella zeae Narita and Hiratsuka) and common rust (caused by Puccinia sorgui Schwein). ND291 has a medium, green plant color and plants have a tendency to develop more than one ear. Inbred trials have shown that ND291 had high seed yield (3.5 t ha^-1), average moisture content at harvest, above average root lodging resistance (1.8%), and above average test weight when compared with 32 elite North Dakota inbreds. ND291 has loose husks and its ears have 14-16 kernel rows. ND291 has intermediate pollen shed and silks two days earlier than ND284 (NDSU, 1998, Carena, 2001), three days earlier than LH176, and four days earlier than A641 in Fargo, ND. Maturity classification is AES 200-300. Breeder seed will be maintained by the North Dakota Agricultural Experiment Station and is distributed upon request (50 kernels per request) from the corresponding author, Plant Sciences Department, North Dakota State University, Fargo, ND 58105. U.S. Plant Variety Protection for ND291 will be requested. H.Z. Cross, D.W. Wanner, and M.J. Carena* References Carena, M.J. 2001. Corn inbred line ‘ND284’. U.S. Plant Variety Protection 9800127. Date issued: 14 May. 2001. Cross, H.Z., and D.W. Wanner. 1991. Registration of NDSAB(MS)C8(LM)C3, NDSD(FS)C1(LM)C4, and NDSM maize germplasms. Crop Sci. 31:239. North Dakota State University. 1998. Corn inbred lines – 1998 releases [Online], Available at http://www.ag.ndsu.nodak.edu/aginfo/seedstock/varieties/VA-CORN.htm#ND284 (verified 31 May. 2002.). Dep. of Plant Sciences, North Dakota State Univ., P.O. Box 5051, Fargo, ND 58105. Registration by CSSA, Accepted . Corresponding author (marcelo.carena@ndsu.nodak.edu). Registration of ND2000 Inbred Line of Maize Inbred ND2000 (Reg. No. PL-306, PI 631394), yellow-dent maize (Zea mays* L.) inbred line, was developed at the North Dakota Agricultural Experiment Station, North Dakota State University (NDSU), Fargo, ND. ND2000 was released 15 February 2002. It has potential to produce early hybrids with high grain yield, low grain moisture at harvest, high test weight, and very good stalk and root lodging resistance in the northern Corn Belt. ND2000 was derived from a synthetic breeding population, NDSCD(M)C8. NDSCD(M)C8 was developed from NDSCD after eight cycles of stratified mass selection for grain yield and standability at low plant densities. NDSCD is a yellow-endosperm synthetic developed by one cycle of full-sib family selection between NDSC(FS)C1 and NDSD(FS)C1 (Cross, 1984, 1988). ND2000 [NDSCD(M)C8-3-2-1-1-1-1] was identified after six generations of selfing during pedigree selection and three years of late generation hybrid testing. Testing of ND2000 was performed in 1999 (four locations), 2000 (five locations), and 2001 (five locations) with commercial check hybrids (2 to 5) included in each trial. Average data across environments showed that two and three-way hybrid combinations with ND2000 (8.15 t ha^-1) had yields that were statistically (P< 0.05) similar to commercial check hybrids (7.77 t ha^-1), and similar root (1.8% vs. 1.5%) and stalk (2% vs. 1.9%) lodging. In addition, ND2000 hybrids showed significant significantly (P< 0.05) lower grain moisture at harvest and higher test weight than check hybrids. ND2000 in hybrid combination had 10 g kg^-1 greater (P< 0.05) protein concentration and similar (P< 0.05) starch and oil concentration than commercial checks. ND2000 has shown good combining ability with early non-Iowa Stiff Stalk Synthetic (BSSS) unrelated commercial and public testers. Simple sequence repeats (SSRs) have suggested that ND2000 could be assigned to the BSSS heterotic group. ND2000 is a vigorous line that has above average resistance to early infection by eyespot (caused by Kabatiella zeae Narita and Hiratsuka) and average resistance to early infection by common rust (caused by Puccinia sorgui Scw.). ND2000 has a medium, green plant color, has heavy pollen shed and tends to produce more than one ear per plant. Its plant height is similar to LH176 in Fargo, ND. ND2000 has shown high seed yield (3.1 t ha^-1) per se and the highest performance index when compared with 35 elite North Dakota inbreds. Inbred trials have also shown that ND2000 had excellent stalk lodging resistance (0.0%). ND2000 has intermediate husk tightness. Its ears have 14 kernel rows and white cobs. ND2000 silks two days earlier than ND286 (NDSU, 1998b, Carena 2002b) and LH176, three days earlier than ND285 (NDSU, 1998a, Carena, 2002a) and A641, and four days earlier than A619 and MBS1236 in Fargo, ND. ND2000 is a good source for developing early and high-yield maturing inbreds. Maturity classification is AES 200-300. Breeder seed will be maintained by the North Dakota Agricultural Experiment Station and is distributed upon request (50 kernels per request) from the corresponding author, Plant Sciences Department, North Dakota State University, Fargo, ND 58105. U.S. Plant Variety Protection for ND2000 will be requested. M.J. Carena* and D.W. Wanner References Carena, M.J. 2002a. Corn inbred line ‘ND285’. U.S. Plant Variety Protection 9800128. Date issued: 20 March. 2002. Carena, M.J. 2002b. Corn inbred line ‘ND286’. U.S. Plant Variety Protection 9800129. Date issued: 19 March. 2002. Cross, H.Z. 1984. Registration of NDSG(MS)C5, NDSC(FS)C1, and NDSD(FS)C1 maize germplasm. Crop Sci. 24:1217. Cross, H.Z. 1988. Registration of NDSCD, NDSK(FS)C1, and NDSL(FS)C1 maize germplasms. Crop Sci. 28:201-202. North Dakota State University. 1998a. Corn inbred lines – 1998 releases [Online], Available at http://www.ag.ndsu.nodak.edu/aginfo/seedstock/varieties/VA-CORN.htm#ND285 (verified 31 May. 2002.). North Dakota State University. 1998b. Corn inbred lines – 1998 releases [Online], Available at http://www.ag.ndsu.nodak.edu/aginfo/seedstock/varieties/VA-CORN.htm#ND286 (verified 31 May. 2002.). Dep. of Plant Sciences, North Dakota State Univ., P.O. Box 5051, Fargo, ND 58105. Registration by CSSA, Accepted . *Corresponding author (marcelo.carena@ndsu.nodak.edu).

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February 5, 2007

TO: NDSU Research Foundation, Agronomy Chairmen and Corn Breeders, North Central Region, and Hybrid Corn Seed Producers

The Director of the North Dakota Agricultural Experiment Station announces the release of six elite inbred lines developed in our corn breeding program.

These lines are the result of an extensive pedigree selection methodology including thousands of lines and over four years of nursery line screening as well as early and late generation hybrid testing across locations, years, and industry testers. Two of the new inbred lines seem promising for use as parents for 70 to 85RM hybrids (Set 1) while four new inbred lines seem promising for use as parents for 85 to 95RM hybrids (Set 2).

Set 1

Experimental inbred ND00-24 is proposed as ND2006. The average performance across locations of single crosses that included ND00-24 as one parent was 13.24% for protein grain content, 56.92 lb/Bu test weight, and 17.46% grain moisture at harvest compared with the average 11.60% for protein grain content, 56.40 lb/Bu test weight, and 18.33% grain moisture at harvest for the commercial check hybrids. It combines best with Iodent and LH82-derived testers. It has shown good combining ability with B14 under drought stress in western ND.

Experimental inbred ND99-8 is proposed as ND2005. The average performance across locations of single crosses that included ND99-8 as one parent was 57.23 lb/Bu test weight, and 17.75% grain moisture at harvest compared with the average, 56.40 lb/Bu for test weight and 18.33% grain moisture at harvest for the commercial check hybrids. Average performance of ND99-8 for other agronomic traits was similar; except ND99-8 crosses had 2.89% less stalk lodging and 9.27% more root lodging. It combines best with Iodent and LH82-derived testers.

Experimental inbred ND01-27 is proposed as ND2004. Average yield across locations of single crosses that included ND01-27 as one parent was 74.1 q ha^-1 (117.85 Bu/A) compared with the average yield of 72.0 q ha^-1 (114.51 Bu/A) for the commercial check hybrids. Average performance of ND01-27 for other agronomic traits was similar, except ND01-27 crosses had 6.2% less root lodging and 3.3% less stalk lodging. It combines well across testers of several heterotic groups.

Experimental inbred ND00-50 is proposed as ND2003. This line had the best specific combining ability for grain yield across locations (80.8 q ha^-1 or 128.43 Bu/A) in combination with commercial tester TR3621Bt especially under irrigated conditions. It combines well across testers of several heterotic groups.

Experimental inbred ND01-32 is proposed as ND2002. Average yield across locations of single crosses that included ND01-27 as one parent was 76.4 q ha^-1 (121.50 Bu/A) compared with the average yield of 72.0 q ha^-1 (114.51 Bu/A) for the commercial check hybrids. Average performance of ND01-32 for other agronomic traits was similar, except ND01-32 crosses had 5.3% greater root lodging and 2.5 lb/Bu less test weight. It combines best with Iodent and LH82-derived testers especially under irrigation.

Experimental inbred ND01-4 is proposed as ND2001. Average yield across locations of single crosses that included ND01-4 as one parent was 74.6 q ha^-1 (118.60 Bu/A) compared with the average yield of 72.0 q ha^-1 (114.51 Bu/A) for the commercial check hybrids. Average performance of ND01-4 for other agronomic traits was similar, except ND01-4 crosses had 2.7 lb/Bu less test weight. It combines best with LH82-derived testers especially in western ND.

Registration of ND291 Inbred Line of Maize

Inbred ND291 (Reg. No. PL-305, PI 631393) is yellow-dent maize (Zea mays L.) inbred line developed at the North Dakota Agricultural Experiment Station, North Dakota State University (NDSU), Fargo, ND. ND291 was released 15 February 2002 because of its potential value as germplasm source in pedigree-selection breeding programs for the northern Corn Belt. ND291 was selected from NDSM(M)C1, a strain of NDSM that has undergone mass selection for increased grain yield and reduced stalk and root lodging. Thirteen inbred lines of approximately AES100 to AES300 maturity were intercrossed to develop NDSM (Cross and Wanner, 1991). These inbred lines were chosen for good general combining ability for grain yield and stalk lodging resistance.

The original S₀ plant was selected from an isolated planting of NDSM(M)C1 and advanced ear-to-row by five generations of self-pollination. ND291 [NDSM(M)C1-1-1-1-1-1-2] was identified in a testcross with an early B14 tester (CM105 x A665) at the S₆ generation. Late-generation testing of ND291 was performed in 1996 (three locations), 1998 (five locations), 1999 (one location), 2000 (six locations), and 2001 (six locations) with commercial check hybrids (2 to 5) included in each trial. The average grain yield of two and three-way hybrid combinations with ND291 (7.45 t ha^-1) across environments were statistically (P< 0.05) similar to commercial check hybrids (7.36 t ha^-1). Crosses that included ND291 had also statistically similar grain moisture at harvest (181.0 g kg^-1 vs. 171.0 g kg^-1), similar root lodging (3.7% vs. 3.2%), and similar stalk lodging (4.0% vs. 3.0%). ND291 hybrids had 10 g kg^-1 greater protein concentration and equal starch and oil concentration than commercial checks. ND291 has shown good combining ability with early Iowa Stiff Stalk Synthetic (BSSS) and unrelated testers. Simple sequence repeats (SSRs) have suggested that ND291 could be assigned to the BSSS heterotic group. Grain yield data, however, do not support molecular data.

ND291 is a vigorous line with above average seedling emergence percentage in cold soils. It has average resistance to early infection by eyespot (caused by Kabatiella zeae Narita and Hiratsuka) and common rust (caused by Puccinia sorgui Schwein). ND291 has a medium, green plant color and plants have a tendency to develop more than one ear. Inbred trials have shown that ND291 had high seed yield (3.5 t ha^-1), average moisture content at harvest, above average root lodging resistance (1.8%), and above average test weight when compared with 32 elite North Dakota inbreds. ND291 has loose husks and its ears have 14-16 kernel rows. ND291 has intermediate pollen shed and silks two days earlier than ND284 (NDSU, 1998, Carena, 2001), three days earlier than LH176, and four days earlier than A641 in Fargo, ND. Maturity classification is AES 200-300.

Breeder seed will be maintained by the North Dakota Agricultural Experiment Station and is distributed upon request (50 kernels per request) from the corresponding author, Plant Sciences Department, North Dakota State University, Fargo, ND 58105. U.S. Plant Variety Protection for ND291 will be requested.

H.Z. Cross, D.W. Wanner, and M.J. Carena*

References

Carena, M.J. 2001. Corn inbred line ‘ND284’. U.S. Plant Variety Protection 9800127. Date issued: 14 May. 2001.

Cross, H.Z., and D.W. Wanner. 1991. Registration of NDSAB(MS)C8(LM)C3, NDSD(FS)C1(LM)C4, and NDSM maize germplasms. Crop Sci. 31:239.

North Dakota State University. 1998. Corn inbred lines – 1998 releases [Online], Available at http://www.ag.ndsu.nodak.edu/aginfo/seedstock/varieties/VA-CORN.htm#ND284 (verified 31 May. 2002.).

Dep. of Plant Sciences, North Dakota State Univ., P.O. Box 5051, Fargo, ND 58105. Registration by CSSA, Accepted . *Corresponding author (marcelo.carena@ndsu.nodak.edu).

Registration of ND2000 Inbred Line of Maize

Inbred ND2000 (Reg. No. PL-306, PI 631394), yellow-dent maize (Zea mays L.) inbred line, was developed at the North Dakota Agricultural Experiment Station, North Dakota State University (NDSU), Fargo, ND. ND2000 was released 15 February 2002. It has potential to produce early hybrids with high grain yield, low grain moisture at harvest, high test weight, and very good stalk and root lodging resistance in the northern Corn Belt. ND2000 was derived from a synthetic breeding population, NDSCD(M)C8. NDSCD(M)C8 was developed from NDSCD after eight cycles of stratified mass selection for grain yield and standability at low plant densities. NDSCD is a yellow-endosperm synthetic developed by one cycle of full-sib family selection between NDSC(FS)C1 and NDSD(FS)C1 (Cross, 1984, 1988).

ND2000 [NDSCD(M)C8-3-2-1-1-1-1] was identified after six generations of selfing during pedigree selection and three years of late generation hybrid testing. Testing of ND2000 was performed in 1999 (four locations), 2000 (five locations), and 2001 (five locations) with commercial check hybrids (2 to 5) included in each trial. Average data across environments showed that two and three-way hybrid combinations with ND2000 (8.15 t ha^-1) had yields that were statistically (P< 0.05) similar to commercial check hybrids (7.77 t ha^-1), and similar root (1.8% vs. 1.5%) and stalk (2% vs. 1.9%) lodging. In addition, ND2000 hybrids showed significant significantly (P< 0.05) lower grain moisture at harvest and higher test weight than check hybrids. ND2000 in hybrid combination had 10 g kg^-1 greater (P< 0.05) protein concentration and similar (P< 0.05) starch and oil concentration than commercial checks. ND2000 has shown good combining ability with early non-Iowa Stiff Stalk Synthetic (BSSS) unrelated commercial and public testers. Simple sequence repeats (SSRs) have suggested that ND2000 could be assigned to the BSSS heterotic group.

ND2000 is a vigorous line that has above average resistance to early infection by eyespot (caused by Kabatiella zeae Narita and Hiratsuka) and average resistance to early infection by common rust (caused by Puccinia sorgui Scw.). ND2000 has a medium, green plant color, has heavy pollen shed and tends to produce more than one ear per plant. Its plant height is similar to LH176 in Fargo, ND. ND2000 has shown high seed yield (3.1 t ha^-1) per se and the highest performance index when compared with 35 elite North Dakota inbreds. Inbred trials have also shown that ND2000 had excellent stalk lodging resistance (0.0%). ND2000 has intermediate husk tightness. Its ears have 14 kernel rows and white cobs. ND2000 silks two days earlier than ND286 (NDSU, 1998b, Carena 2002b) and LH176, three days earlier than ND285 (NDSU, 1998a, Carena, 2002a) and A641, and four days earlier than A619 and MBS1236 in Fargo, ND. ND2000 is a good source for developing early and high-yield maturing inbreds. Maturity classification is AES 200-300.

M.J. Carena* and D.W. Wanner

References

Carena, M.J. 2002a. Corn inbred line ‘ND285’. U.S. Plant Variety Protection 9800128. Date issued: 20 March. 2002.

Carena, M.J. 2002b. Corn inbred line ‘ND286’. U.S. Plant Variety Protection 9800129. Date issued: 19 March. 2002.

Cross, H.Z. 1984. Registration of NDSG(MS)C5, NDSC(FS)C1, and NDSD(FS)C1 maize germplasm. Crop Sci. 24:1217.

Cross, H.Z. 1988. Registration of NDSCD, NDSK(FS)C1, and NDSL(FS)C1 maize germplasms. Crop Sci. 28:201-202.

North Dakota State University. 1998a. Corn inbred lines – 1998 releases [Online], Available at http://www.ag.ndsu.nodak.edu/aginfo/seedstock/varieties/VA-CORN.htm#ND285 (verified 31 May. 2002.).

North Dakota State University. 1998b. Corn inbred lines – 1998 releases [Online], Available at http://www.ag.ndsu.nodak.edu/aginfo/seedstock/varieties/VA-CORN.htm#ND286 (verified 31 May. 2002.).

Dep. of Plant Sciences, North Dakota State Univ., P.O. Box 5051, Fargo, ND 58105. Registration by CSSA, Accepted . *Corresponding author (marcelo.carena@ndsu.nodak.edu).