The Effect of Rumen Degradable and Undegradable Protein
Supplementation in Barley-Based High-Grain Finishing Diets on Feedlot
Performance and Carcass Traits of Beef Steers
Benjamin Pamp, Marc Bauer, Greg Lardy
NDSU Department of Animal and Range
Sciences
Objectives were to evaluate the use of increased
ruminally degradable (RDP) and ruminally undegradable protein (RUP) source on
performance and carcass traits of finishing steer calves. Because of the high degradability of barley
protein, the addition of RDP to the diet did not have a significant effect on
performance or carcass traits. However,
both performance and carcass traits were improved by the inclusion of RUP.
One hundred and forty-one crossbred steers (972 ± 16 lbs. initial BW) were allotted randomly to one of four dietary
treatments (6 pens/treatment) in a completely
randomized design and fed for 89 days.
Initial weights were determined by a 3-day average during which steers
were fed a common diet at 2 percent of BW.
Dietary treatments and diet formulations were arranged in a 2 x 2
factorial. Factors were RDP (+/-) and
RUP (+/-). Diets were formulated such
that +RDP added 1 percent CP from urea and +RUP added 1% CP from the feather
meal/blood meal combination. The basal
diet was formulated to contain (DM basis) 83 percent barley, 5 percent alfalfa
hay, 5 percent corn silage, 5 percent desugared molasses, 2
percent supplement, 25 g/ton monensin, and 10 g/ton tylosin, and was formulated
to contain a minimum of 12.5 percent CP, 0.7 percent Ca, 0.3 percent P, and 0.7
percent K. Steers were fed once daily
and weighed at 28-day intervals. Final
weight was not affected by dietary treatment (P = 0.18); however, ADG tended (P
= 0.08) to increase with RUP supplementation.
The dietary treatments had no affect on DMI (P = 0.74), gain efficiency
(P = 0.15), or hot carcass weight (P = 0.18).
There was an RDP x RUP interaction (P = 0.02) for ribeye area which
increased with RUP supplementation when RDP was not supplemented (13.6 vs. 12.1
+ 0.2 in2); however, when RDP was supplemented RUP supplementation
did not effect REA (13.6 vs. 12.7 + 0.2 in2). Supplemental RUP also decreased (P = 0.01)
calculated yield grade (2.13 vs. 2.44 + 0.05).
Supplemental RDP did not improve performance or carcass characteristics
(P > 0.35). It appears that RDP may
be adequate in barley-based finishing diets; however, supplemental RUP may
improve performance in steers fed barley-based high grain diets.
Introduction
Protein consumed by ruminants is divided into RDP
(ruminally degraded protein) and RUP (ruminally undegraded
protein). Degradable protein is used by
ruminal microbes, to convert feed nutrients to microbial protein, rather than
by the animal itself. Ruminally degraded
protein can come in the form of NPN (non-protein nitrogen) and true protein,
which supplies the rumen microbes with amino acids and peptides. These nutrients are used by the microbes to
support microbial fermentation. A deficiency
in RDP would result in reduced carbohydrate digestion, VFA and microbial
protein production. This would decrease
animal performance.
A study was conducted to determine RDP requirement of
finishing steers fed steam-flaked corn (Cooper et al., 2002). Cooper et al.(2002)
found that as dietary RDP increased feed/gain and average daily gain responded
quadratically. Dry matter intake also
increased quadratically in response to increased RDP. Little research has been done with
supplementation of RDP in barley-based finishing diets. Barley is higher in total protein than corn and
has a much higher portion of total protein being degradable in the rumen than
corn. The non-structural carbohydrates
contained in barley are more quickly and completely fermented than those in
corn, perhaps increasing the need for RDP.
This raises the question as to whether RDP and/or RUP supplementation
are needed in barley-based finishing.
Procedures
One hundred and forty-one crossbred steers (972.4 " 0.4 lb. initial BW) were allotted randomly to one of
four dietary treatments (6 pens/treatment). Initial weights were determined by a 3-day
average during which steers were fed a common diet at 2 percent of BW. The control diet (without added RDP and RUP)
was formulated to contain a minimum of 12.5 percent CP, 0.7 percent calcium,
0.3 percent phosphorus, 0.7 percent potassium, and .25 percent salt. Diets containing added RDP (0.37% urea) added
one percentage point more CP and diets containing added RUP (1.39% feather meal
and 0.35% blood meal) added one percentage point more CP (Table 1). Protein sources replaced barley.
Steers were fed once daily
for 89 days and weighed at 28 day intervals; final weights were calculated from
hot carcass weight at 62 percent yield.
Intakes were recorded daily. Diet
samples were collected, weighed, and sampled for DM weekly.

a Diets were
formulated such that +RDP added 1% CP from urea and +RUP added 1% CP from the
feather meal/blood meal combination.
1
Concentrated separator by-product (desugared molasses).
2 Contained
copper (6183 mg/lb), iron (9275 mg/lb), iodine (568 mg/lb),
manganese (26282 mg/lb), 17600 kIU/kg
vitamin A, 1397 kIU/kg vitamin D, 7.04 kIU/kg vitamin E,
monensin at 176.4 g/kg premix, tylosin at 88.2 g/kg premix,
salt, and limestone.
Results
Final weight was not affected by dietary treatment (P
= 0.18); however, although not significant, final weight tended to be slightly
higher on average for the RUP treatment than for other treatments with
supplemental protein and almost 37 lbs. higher than the control group.
Average daily gain increased (P = 0.08) with RUP
supplementation. The dietary treatments
had no affect on DMI (P = 0.74). Feed
efficiency tended to increase (P = 0.15) with RUP supplementation both with and
without the addition of RDP. Hot carcass
weight (HCW) also was not affected by treatment (P $ 0.18).
There was an RDP x RUP interaction (P = 0.02) for
ribeye area which increased with RUP supplementation when RDP was not
supplemented (13.6 vs. 12.1 ± 0.2 in2); however,
when RDP was supplemented RUP supplementation did not effect REA (13.6 vs. 12.7
± 0.2 in2).
Supplemental RUP also
decreased (P = 0.01) calculated yield grade (2.13 vs. 2.44 ± 0.05). Supplemental RDP did not
improve performance or carcass characteristics (P > 0.35).
Note: This
study is part of a collaborative research project that includes Animal and
Range Sciences Department and the Carrington Research Extension Center.

a Diets were formulated such that +RDP added 1% CP from
urea and +RUP added 1% CP from the feather meal/blood meal combination.
b Standard error of the mean
c ADG/DMI
d 300 = slight0, 400 = small0, and
500 = modest0 marbling
Conclusions
Supplementing a barley-based finishing diet with RUP
tended to increase gain and improved yield grade. Supplemental RDP did not improve performance
or carcass characteristics.
Implications
These results suggest that barley grain is a suitable
ingredient in finishing feedlot diets. Also, barley-based feedlot finishing
diets may contain adequate RDP, but RUP is deficient and might need to be
supplemented.
Literature
Cited
Cooper, R.J., C.T. Milton, T.J.
Klopfenstein, and D.J. Jordon.
2002. Effect of corn processing on
degradable intake protein requirement of finishing cattle. J. Anim.Sci. 80:242-247.